Inscrit le: 10 Oct 2017
|Posté le: Lun 11 Déc 2017 - 05:48 Sujet du message: Paralens fluorescent attachment to detect DAPI and Hoech
From what I can see, I don't suppose it will work with DAPI considering the setup. That filter set is meant to be used with FITC (blue excitation, green emission) as opposed to DAPI (UV excitation, blue emission). It will also work with red-emitting fluorocromes, if their excitation range matches the setup.
It is true that the excitation filter would allow light in the 385-480nm range to pass, but the LED will only emit light at 410nm and above.
At page 1 (5th page of the PDF), the manual states, "The ParaLens Advance’s blue LED light source attaches to the filter set arm, emitting blue light with a 410-511 nm wavelength" (That would be "light output" on the figure at page 9).
Also, the dichroic mirror and emission filter setup are "in practice ok" (could be better) for emission wavelengths of 525-530nm and below, which again is OK for FITC, but it'll cut out any blue light the specimen might produce.
I didn't find the right solution from the internet.
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